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human embryonic diploid lung fibroblast helf cell line human fetal lung fibroblast 1  (ATCC)


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    ATCC human embryonic diploid lung fibroblast helf cell line human fetal lung fibroblast 1
    Human Embryonic Diploid Lung Fibroblast Helf Cell Line Human Fetal Lung Fibroblast 1, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 663 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human embryonic diploid lung fibroblast helf cell line human fetal lung fibroblast 1/product/ATCC
    Average 96 stars, based on 663 article reviews
    human embryonic diploid lung fibroblast helf cell line human fetal lung fibroblast 1 - by Bioz Stars, 2026-03
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    human embryonic diploid lung fibroblast helf cell line human fetal lung fibroblast 1  (ATCC)
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    Embryonic lung <t>fibroblasts</t> stimulate the differentiation of lung progenitors into HT-280 + AEC2 cells. A Top panel: Schematic of the indirect Transwell® co-culture system to determine the effect of irradiated human embryonic lung fibroblasts (ihELF) on the differentiation of D22 NKX2-1 + LPCs into AEC2. Bottom panel: Flow cytometry analysis for CDH1 and HT2-280 of organoids grown in different matrices. Data are expressed as mean ± SEM, n = 4 biological replicates. Dashed lines indicate mean % of HT2-280 positive cells, respectively, after combining the matrix data in both groups. * p < 0.05 by Mann–Whitney U test, no ihELF versus ihELF. B Left panel: Schematic of experimental design to determine the effect of direct versus indirect contact of LPCs with ihELF on the differentiation of D22 NKX2-1 + LPCs to AEC2. Right panel: Flow cytometry analysis for HT2-280 of organoids grown directly versus indirectly with ihELF after 20 days of culture. Data are expressed as mean ± SEM, n ≥ 10 biological replicates
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    Embryonic lung <t>fibroblasts</t> stimulate the differentiation of lung progenitors into HT-280 + AEC2 cells. A Top panel: Schematic of the indirect Transwell® co-culture system to determine the effect of irradiated human embryonic lung fibroblasts (ihELF) on the differentiation of D22 NKX2-1 + LPCs into AEC2. Bottom panel: Flow cytometry analysis for CDH1 and HT2-280 of organoids grown in different matrices. Data are expressed as mean ± SEM, n = 4 biological replicates. Dashed lines indicate mean % of HT2-280 positive cells, respectively, after combining the matrix data in both groups. * p < 0.05 by Mann–Whitney U test, no ihELF versus ihELF. B Left panel: Schematic of experimental design to determine the effect of direct versus indirect contact of LPCs with ihELF on the differentiation of D22 NKX2-1 + LPCs to AEC2. Right panel: Flow cytometry analysis for HT2-280 of organoids grown directly versus indirectly with ihELF after 20 days of culture. Data are expressed as mean ± SEM, n ≥ 10 biological replicates
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    Embryonic lung <t>fibroblasts</t> stimulate the differentiation of lung progenitors into HT-280 + AEC2 cells. A Top panel: Schematic of the indirect Transwell® co-culture system to determine the effect of irradiated human embryonic lung fibroblasts (ihELF) on the differentiation of D22 NKX2-1 + LPCs into AEC2. Bottom panel: Flow cytometry analysis for CDH1 and HT2-280 of organoids grown in different matrices. Data are expressed as mean ± SEM, n = 4 biological replicates. Dashed lines indicate mean % of HT2-280 positive cells, respectively, after combining the matrix data in both groups. * p < 0.05 by Mann–Whitney U test, no ihELF versus ihELF. B Left panel: Schematic of experimental design to determine the effect of direct versus indirect contact of LPCs with ihELF on the differentiation of D22 NKX2-1 + LPCs to AEC2. Right panel: Flow cytometry analysis for HT2-280 of organoids grown directly versus indirectly with ihELF after 20 days of culture. Data are expressed as mean ± SEM, n ≥ 10 biological replicates
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    human embryonic lung fibroblast helf  (ATCC)
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    ATCC human embryonic lung fibroblast helf
    Embryonic lung <t>fibroblasts</t> stimulate the differentiation of lung progenitors into HT-280 + AEC2 cells. A Top panel: Schematic of the indirect Transwell® co-culture system to determine the effect of irradiated human embryonic lung fibroblasts (ihELF) on the differentiation of D22 NKX2-1 + LPCs into AEC2. Bottom panel: Flow cytometry analysis for CDH1 and HT2-280 of organoids grown in different matrices. Data are expressed as mean ± SEM, n = 4 biological replicates. Dashed lines indicate mean % of HT2-280 positive cells, respectively, after combining the matrix data in both groups. * p < 0.05 by Mann–Whitney U test, no ihELF versus ihELF. B Left panel: Schematic of experimental design to determine the effect of direct versus indirect contact of LPCs with ihELF on the differentiation of D22 NKX2-1 + LPCs to AEC2. Right panel: Flow cytometry analysis for HT2-280 of organoids grown directly versus indirectly with ihELF after 20 days of culture. Data are expressed as mean ± SEM, n ≥ 10 biological replicates
    Human Embryonic Lung Fibroblast Helf, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human embryonic lung fibroblast helf/product/ATCC
    Average 99 stars, based on 1 article reviews
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    human embryonic lung fibroblasts helf  (ATCC)
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    ATCC human embryonic lung fibroblasts helf
    Embryonic lung <t>fibroblasts</t> stimulate the differentiation of lung progenitors into HT-280 + AEC2 cells. A Top panel: Schematic of the indirect Transwell® co-culture system to determine the effect of irradiated human embryonic lung fibroblasts (ihELF) on the differentiation of D22 NKX2-1 + LPCs into AEC2. Bottom panel: Flow cytometry analysis for CDH1 and HT2-280 of organoids grown in different matrices. Data are expressed as mean ± SEM, n = 4 biological replicates. Dashed lines indicate mean % of HT2-280 positive cells, respectively, after combining the matrix data in both groups. * p < 0.05 by Mann–Whitney U test, no ihELF versus ihELF. B Left panel: Schematic of experimental design to determine the effect of direct versus indirect contact of LPCs with ihELF on the differentiation of D22 NKX2-1 + LPCs to AEC2. Right panel: Flow cytometry analysis for HT2-280 of organoids grown directly versus indirectly with ihELF after 20 days of culture. Data are expressed as mean ± SEM, n ≥ 10 biological replicates
    Human Embryonic Lung Fibroblasts Helf, supplied by ATCC, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human embryonic lung fibroblasts helf/product/ATCC
    Average 97 stars, based on 1 article reviews
    human embryonic lung fibroblasts helf - by Bioz Stars, 2026-03
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    		  Buy from Supplier
    human embryonic lung fibroblasts helfs  (ATCC)
    97
    ATCC human embryonic lung fibroblasts helfs
    Embryonic lung <t>fibroblasts</t> stimulate the differentiation of lung progenitors into HT-280 + AEC2 cells. A Top panel: Schematic of the indirect Transwell® co-culture system to determine the effect of irradiated human embryonic lung fibroblasts (ihELF) on the differentiation of D22 NKX2-1 + LPCs into AEC2. Bottom panel: Flow cytometry analysis for CDH1 and HT2-280 of organoids grown in different matrices. Data are expressed as mean ± SEM, n = 4 biological replicates. Dashed lines indicate mean % of HT2-280 positive cells, respectively, after combining the matrix data in both groups. * p < 0.05 by Mann–Whitney U test, no ihELF versus ihELF. B Left panel: Schematic of experimental design to determine the effect of direct versus indirect contact of LPCs with ihELF on the differentiation of D22 NKX2-1 + LPCs to AEC2. Right panel: Flow cytometry analysis for HT2-280 of organoids grown directly versus indirectly with ihELF after 20 days of culture. Data are expressed as mean ± SEM, n ≥ 10 biological replicates
    Human Embryonic Lung Fibroblasts Helfs, supplied by ATCC, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human embryonic lung fibroblasts helfs/product/ATCC
    Average 97 stars, based on 1 article reviews
    human embryonic lung fibroblasts helfs - by Bioz Stars, 2026-03
    97/100 stars
    		  Buy from Supplier

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    Embryonic lung fibroblasts stimulate the differentiation of lung progenitors into HT-280 + AEC2 cells. A Top panel: Schematic of the indirect Transwell® co-culture system to determine the effect of irradiated human embryonic lung fibroblasts (ihELF) on the differentiation of D22 NKX2-1 + LPCs into AEC2. Bottom panel: Flow cytometry analysis for CDH1 and HT2-280 of organoids grown in different matrices. Data are expressed as mean ± SEM, n = 4 biological replicates. Dashed lines indicate mean % of HT2-280 positive cells, respectively, after combining the matrix data in both groups. * p < 0.05 by Mann–Whitney U test, no ihELF versus ihELF. B Left panel: Schematic of experimental design to determine the effect of direct versus indirect contact of LPCs with ihELF on the differentiation of D22 NKX2-1 + LPCs to AEC2. Right panel: Flow cytometry analysis for HT2-280 of organoids grown directly versus indirectly with ihELF after 20 days of culture. Data are expressed as mean ± SEM, n ≥ 10 biological replicates

    Journal: Stem Cell Research & Therapy

    Article Title: Influence of mesenchymal and biophysical components on distal lung organoid differentiation

    doi: 10.1186/s13287-024-03890-2

    Figure Lengend Snippet: Embryonic lung fibroblasts stimulate the differentiation of lung progenitors into HT-280 + AEC2 cells. A Top panel: Schematic of the indirect Transwell® co-culture system to determine the effect of irradiated human embryonic lung fibroblasts (ihELF) on the differentiation of D22 NKX2-1 + LPCs into AEC2. Bottom panel: Flow cytometry analysis for CDH1 and HT2-280 of organoids grown in different matrices. Data are expressed as mean ± SEM, n = 4 biological replicates. Dashed lines indicate mean % of HT2-280 positive cells, respectively, after combining the matrix data in both groups. * p < 0.05 by Mann–Whitney U test, no ihELF versus ihELF. B Left panel: Schematic of experimental design to determine the effect of direct versus indirect contact of LPCs with ihELF on the differentiation of D22 NKX2-1 + LPCs to AEC2. Right panel: Flow cytometry analysis for HT2-280 of organoids grown directly versus indirectly with ihELF after 20 days of culture. Data are expressed as mean ± SEM, n ≥ 10 biological replicates

    Article Snippet: Human embryonic lung fibroblasts (hELF, 17.5 weeks of gestation, DV Biologics) were cultured in DMEM (Gibco, #11965118) containing 10% (v/v) FBS and 1% (v/v) penicillin/streptomycin.

    Techniques: Co-Culture Assay, Irradiation, Flow Cytometry, MANN-WHITNEY